WGS (Whole Genome Sequencing) Abbreviation
What is WGS?
WGS stands for Whole Genome Sequencing, a method that determines the complete DNA sequence of an organism's genome. In microbiome research, shotgun metagenomics is sometimes called "metagenomic WGS."
Applications
Single Organism WGS
- Complete genome of one bacterium, virus, or other organism
- Used for strain identification and characterization
Shotgun Metagenomics (Metagenomic WGS)
- Sequences all DNA in a complex sample
- Captures entire microbial community
- Provides both taxonomic and functional information
Advantages Over 16S
| Feature | 16S rRNA | Shotgun WGS |
|---|---|---|
| Resolution | Genus/species | Strain-level |
| Functional Genes | No | Yes |
| Non-bacterial | Limited | Viruses, fungi, archaea |
| Cost | Lower | Higher |
| Depth Required | Lower | Higher |
Data Generated
- Taxonomic Profiles: Species and strain identification
- Functional Annotations: Metabolic pathways, antibiotic resistance genes
- Genomic Features: Plasmids, mobile elements
Related Terms
- Metagenomics: Study of genetic material from environmental samples
- 16S rRNA: Targeted bacterial sequencing
- Sequencing Technology: Platforms used for WGS