Metagenomics Data type
What is Metagenomics?
Metagenomics is the study of genetic material recovered directly from environmental or clinical samples. Unlike traditional genomics that sequences individual organisms, metagenomics sequences all DNA in a sample, capturing the entire microbial community.
How It Works
- Sample Collection: Collect sample (stool, saliva, soil, etc.)
- DNA Extraction: Extract all DNA from the sample
- Sequencing: Sequence the DNA using high-throughput methods
- Bioinformatics: Analyze sequences to identify organisms and genes
Types of Metagenomic Analysis
Targeted Sequencing (Amplicon)
- 16S rRNA: Identifies bacteria and archaea
- 18S rRNA: Identifies eukaryotes
- ITS: Identifies fungi
Whole Metagenome Sequencing (Shotgun)
- Sequences all DNA without targeting specific genes
- Provides functional gene information
- Higher resolution for species identification
Applications in CMMI-DCC
- Microbiome Profiling: Identify gut microbiome composition
- Functional Analysis: Discover microbial metabolic capabilities
- Disease Associations: Link microbiome changes to health outcomes
- Multi-Omics Integration: Combine with metabolomics data
Data in CMMI-DCC
Metagenomics results include:
- Taxonomic profiles (which organisms are present)
- Abundance data (how much of each organism)
- Sequencing technology and quality metrics
Related Terms
- Microbiome: The community of microorganisms
- 16S rRNA: Bacterial identification method
- WGS: Whole Genome Sequencing
- Alpha Diversity: Within-sample diversity measure